The clip shows manager Christine Thompson and two staff sharing some wine at the end of a shift.
Staff believe the act could be the ghost of a late school mistress said to haunt the pub which used to be a school.
Barmaid Elaine Drabble can be seen taking a sip before setting her glass back on the table.
Incredibly, the glass then seems to move of its own accord towards the women.
It teeters on the edge of the table before tumbling into Elaine’s lap.
Ms Thompson, 61, is convinced a ghost was behind the mysterious happening.
She believes the “spirit” may have been angered after Elaine had described a book she was reading about the horrors of the Nazi regime.
She said: “On the camera as you watch the footage, you can see her glass starts to move.
“I said to Elaine, ‘I think the spirit didn’t like the subject you were talking about’.”
The landlady thinks the supposed ghost is a school mistress which has been haunting the pub since Victorian times, when the drinking spot was used as a classroom.
There have been sightings of the “spirit” in the past, with many describing it as a woman in a long, black skirt with lace-up boots, Dailystar.co.uk reports.
Ms Thompson even has a historical photo of two mistresses – from Mosborough Endowed School – in the same attire.
She recalled how, in 1998, a group of builders spotted it when they were extending a function room.
She said: “That particular morning, two guys were upstairs with massive hammers hitting the stone walls to knock a doorway through.
“Next thing I know they came flying down the staircase – two burly builders in their 40s with tattoos and massive shoulders on them – not the sort of guys you would think scare very easily.”
Another drinker who visited the pub told Christine she had encountered the ghost while working there two decades before.
Ms Thompson said: “She said ‘the woman I saw in the kitchen, she had a long black skirt on down to her calves, and black lace-up boots’.
“It was exactly the same description.”
The landlady also believes that a photo taken in her cellars by a local history group captures the faces of four ghosts.
She said: “Honestly, I can see four faces in this photograph – as clear as day.
“To me they’ve got to be ghosts. They can’t be anything else, because there was nothing down there [when they were taken] – just us.”
There is no scientific proof of ghosts, poltergeists or the afterlife, but thousands of people believe tortured souls can remain on in this world as spooks if they have unfinished business or if they suffered a particularly violent or traumatic death.